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Objective To detect the association between rs4646999 polymorphisms in the promoter region of the c-Jun and the prognosis of sporadic colorectal cancer. Methods rs4646999-673C>T genetypes were deter-mined by Taqman-MGB probes in 436 colorectal cancer cases. The survival curve was analyzed by Kaplan-Meier analysis and Cox regression.Western blot was used to analyze the expression levels of c-Jun protein in different gen-otypes. Results Univariate analysis showed that the cumulative survival rate of patients with rs4646999TT geno-type was significantly higher than that of patients with CT and CC genotype. Multivariate Cox regression analysis showed that the differentiation,lymph node metastasis,distant metastasis,TNM stage and rs4646999 genetypes were prognostic factors.Compared with the carriers of TT genotype,CT/CC complex genotypes were associated with poor prognosis of colorectal cancer(P<0.05).Protein expression analysis showed that the expression of c-Jun pro-tein in CC genotype was increased.In contrast,the TT genotype was decreased.Conclusions This study provided the evidence that rs4646999-673C>T genetic variant in c-Jun promoter regions is associated with the poor survival prognosis of colorectal cancer,possibly by elevating the protein expression levels that appeared to up-regulate activ-ity of c-Jun thus tumorigenesis.
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Objective To study the effect of CO2 pneumoperitoneum on the anti-apoptotic capability of human cervical carcinoma cell line(CaSki).Methods The imitated CO2 pneumoperitoneum was established in vitro.After CaSki cells were exposed to CO2 pneumoperitoneum at 0,7 or 14 mmHg for 1,2,3,4 h,the expressions of Survivin mRNA and Bcl-2 protein were examined by RT-PCR and Western blot respectively.The untreated CaSki cells served as control.Results Under 7 or 14 mmHg,the expressions of Survivn and Bcl-2 had an increasing tendency with the exposure time prolongation.Within the same exposure time,the expressions of Survivin and Bcl-2 under 7 mmHg were significantly higher than those under 0 or 14 mmHg or in control group.Conclusion Under certain pressure and within certain exposure time,CO2 pneumoperitoneum could improve anti-apoptotic capability of human cervical carcinoma cells,which may be one reason for the promoting effect of carbon dioxide pneumoperitoneum on malignant cells growth.
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0.05),but at 72h,96h,120h after exposure,the absorbance values in carbon dioxide pneumoperitoneum group were always significantly higher than those in control group at the same time(P0.05).Conclusions:The model established with a special airtight container and an automatic pneumoperitoneum meter is an ideal one for research about laparoscopy in vitro,and the carbon dioxide pneumoperitoneum in this model increases the in vitro growth of human cervical carcinoma cells.
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Objective:To evaluate the effects of different pneumoperitoneums used in laparoscopy on the growth of human cervical carcinoma cells in vitro.Methods:Human cervical carcinoma cell line(CaSki)were cultured in vitro and then exposed to pneumoperitoneums of pure carbon dioxide or pure nitrogen gas with the untreated group as control. The cell growth was determined by MTT assay,flow cytometry and electron microscopy. Results:Carbon dioxide stimulated the cell growth from 48h to 120h after treatment. For nitrogen gas group,there was a transient descent of cell proliferation from 48h to 72h after treatment. The tumor cells of carbon dioxide group in G2-M phases significantly increased. Active karyokinesis was observed in carbon dioxide group while slight mitochondrion swelling was observed in nitrogen gas group with the nucleus space expanded. Conclusions:Carbon dioxide pneumoperitoneum could increase the growth of human cervical carcinoma cells in vitro whereas nitrogen gas pneumoperitoneum had inhibiting effect on the cell growth.